AmScope IN300TC-FL-5MF Digital Long Working Distance Inverted Fluorescence Trinocular Microscope, 40x-1000x, WH10x and WH25x Eyepieces, Phase-Contrast Objectives, 30W Halogen Illumination, 0.3 NA Abbe Condenser, Mechanical Stage, 110V, Includes 5MP Peltie

The AmScope IN300TC-FL-5MF digital inverted epifluorescence trinocular microscope has interchangeable pairs of 10x22mm and 25x10mm high-eyepoint super-widefield eyepieces, an under-mounted quadruple nosepiece with four DIN long working-distance plan objectives, fluorescence 100W HBO mercury lamp and Brightfield 30W halogen illumination, a 0.3 NA Abbe condenser with iris diaphragm, and a double-layer mechanical stage with a stage stop to protect slides and objectives from damage. The 5MP Peltier-cooled fluorescence camera has a CMOS color sensor, image capture and editing software, and USB 2.0 output to capture or display still or video images on a computer or projector. The anti-mold trinocular head has a Siedentopf binocular mount with 50 to 75mm interpupillary adjustment and a fixed 45-degree vertical inclination to reduce eye and neck strain. A Siedentopf binocular head enables the viewer to change the interpupillary distance without changing the tube length, eliminating the need to refocus the image. Anti-mold coatings protect the microscope in high-humidity environments. Interchangeable pairs of WH10x22mm and WH25x10mm high-eyepoint super-widefield eyepieces provide improved focus over the entire field of view (FOV). High-eyepoint eyepieces ease viewing for viewers who wear glasses. Dioptric adjustment on both eyepieces accommodates individual eye-strength differences. The vertical trinocular port accepts a 23mm or C-Mount camera. The under-mounted nosepiece has 4x, 10xPH (phase-contrast), 20xPH (phase-contrast), and 40x (spring) DIN long working-distance plan objectives that combine with the eyepieces to provide color correction of magnified images. Long working-distance plan objectives provide improved focus over the entire range of the viewing field and a longer working distance that is required when viewing specimens in dishes and large containers. Phase-contrast provides high contrast and visibility without the use of stains, allowing specimens to be observed in their natural state without being killed or fixed. A digital inverted microscope is used in research, medical, and industrial laboratories for observation of biological specimens within vials or dishes that require more vertical working space, and where image capture, detailed records, or documentation is required.

The included AmScope MT5000-CCD 5.0MP (2580 x 1944 pixels) Peltier-cooled fluorescence camera has a 2/3" Sony color CCD sensor for displaying still microscopy images and streaming live videos to a computer monitor or digital TV. The camera can be mounted in any standard C-Mount. The Peltier-cooled sensor provides low-noise fluorescence image acquisition. The sensor has an interline scan mode, an RGB Bayer pattern filter, and a 280mV G-sensitivity. The camera includes image capture and editing software that provides still image and live video capture and editing capability, including measurement functions. Users can select between 2580 x 1944 or 1280 x 932 display resolution. User-defined parameters include brightness, gain, automatic or manual exposure selection, RGB, white balance, and light angle. Exposure duration has a range of 0.1 to 60 minutes. The software supports JPG, TIFF, GIF, PSD, WMF, and BMP file formats and is compatible with Windows XP, Vista, 7, and 8. The camera supports Twain, DirectShow, Amcap, and Minisee file formats. The camera has a USB 2.0 data port (cable included).

Brightfield, phase-contrast, and fluorescence illumination is mounted above the stage and has transmitted 30W halogen illumination. Brightfield (BF) illumination allows the specimen to absorb light, resulting in a dark image on a light background. The phase-contrast element aligns the light source with the phase-contrast objectives to optimize high contrast and visibility without the use of stains, allowing specimens to be observed in their natural state without being killed or fixed. Halogen illumination provides bright light in a concentrated path, and a rheostat controls the amount of light emanating from the lamp. Often used in biological and metallurgical research, fluorescence microscopy uses a xenon or mercury lamp to create ultraviolet light and excitation filters to filter unwanted wavelengths, allowing users to see details undetectable to other light sources. The movable 0.3 NA Abbe condenser can be adjusted to control the distance of the light from the stage, and the iris diaphragm has an opening that focuses and controls the amount of light illuminating the specimen from fully open to fully closed. The double-layer mechanical stage with 1mm stage divisions and 0.1mm vernier resolution locks the slide into place and provides precise slide manipulation along the X- and Y-axes to allow coordinates to be recorded, enabling the viewer to return to a specific location on the slide. The oversize stage is 9.53 x 6.77 inches (242 x 172mm) (W x D, where W is width, the horizontal distance from left to right; D is depth, the horizontal distance from front to back). Nested coaxial coarse and fine focus has tension-adjustable coarse focusing and an adjustable lock ring stopper that limits stage range to protect slides and objectives from damage. All-metal mechanical parts, solid-metal frame construction, and a stain-resistant enamel finish provide durability.

Microscopes are instruments used to enhance the resolution of an object or image. Types include compound, stereo, or digital. Compound microscopes use a compound optical system with an objective lens and an eyepiece. Stereo microscopes show object depth in a three-dimensional image. Digital microscopes are used to display an image on a monitor, rather than looking through a lens. Microscopes can have monocular (one), binocular (two), or trinocular (three) eyepieces, with varying magnification abilities. Magnification ability refers to the size of an image. Resolution, also known as resolvant power, refers to the clarity of the image. The interaction between field of view (FOV), numerical aperture (NA), and working distance (WD) determines resolution. Microscopes can control magnification through a fixed focus, or through a range of adjustments. They can also utilize LED, fluorescent, and mirror light sources to help control viewing capabilities. Microscopes are widely used in education, lab research, biology, metallurgy, engineering, chemistry, manufacturing, and in the medical, forensic science, and veterinary industries.

United Scope manufactures microscopy equipment and accessories under the brand name AmScope. The company, founded in 1996, is headquartered in Irvine, CA.

What's in the Box?

• AmScope IN300TC-FL-5MF microscope
• WH10x20mm plan eyepieces, 30mm, one pair
• WH25Sx10mm plan eyepieces, 30mm, one pair
• 4x DIN long working-distance plan objective, 20mm
• 10x DIN long working-distance phase-contrast plan objective, 20mm
• 20x DIN long working-distance phase-contrast plan objective, 20mm
• 40xS DIN long working-distance plan objective, 20mm
• MT5000-CCD 5MP CMOS camera
• Peltier-cooled cable
• Peltier-cooled power supply
• (3) Color filters; yellow, blue, and green
• Reflected illuminations system with top light housing and electrical cord
• Episcopic light box and tube
• Fluorescence Illumination power box
• Mercury bulb, 100W
• Halogen bulb, 30W
• Protecting filter
• USB cable
• Software CD
• Aluminum case
• Power cord
• Dust cover
• Instructions